Example 1: Multi-Level Ensemble Refinement๏ƒ

This tutorial reproduces the Ensemble Refinement case study described in the paper. The goal is to refine a large ensemble of a flexible molecule generated by CREST (or any other conformational search tool existing).

This command launches the refinement process using 8 CPU cores. The -e flag specifies the input ensemble, and -p points to our custom JSON protocol.

ensemble_analyzer -e ensemble.xyz -p protocol_refinement.json -cpu 8
{
    "0": {
        "functional": "ExtOpt",
        "basis": "def2-mTZVP", 
        "add_input": "\n%method ProgExt '/path/to/orca-external-tools/gxtb.py' end\n"
    },
    "1": {
        "functional": "r2SCAN-3c", 
        "basis": "def2-mTZVPP", 
        "opt": true, 
        "freq": true,
        "skip_opt_fail": true       
    },
    "2":{
        "functional": "wB97X-D4rev",
        "basis": "def2-QZVPP",
        "add_input": "\n! tightscf defgrid3\n", 
        "read_orbitals": 1
    }
}
  20
        -41.34883243
 O         -0.1748654122       -0.9626180924        0.6834858478
 O         -1.2606630407        0.5916728012       -1.4724067115
 O          1.1330578318        2.3464327344        0.3656965439
 O         -2.9346429807       -1.0551940920       -0.0928149187
 O          3.0209489612        0.0960169965       -0.5377443649
 O          1.4324099664       -2.3609273744       -0.0637489018
 C         -0.3604058709        0.4450086523        0.7841967393
 C         -1.5247105068        0.9171760702       -0.1378351876
 C          0.9500351433        1.0282873556        0.3469579105
 C         -2.8600456175        0.2915715899        0.3056510218
 C          1.7548670973        0.0186063615       -0.0472781181
 C          1.0462326418       -1.2331909281        0.1608222098
 H         -0.6022819098        0.6952476715        1.8219130421
 H         -1.5618451573        2.0085918963       -0.0592783048
 H         -2.9936544203        0.4034794918        1.3867384527
 H         -3.6783742131        0.7968196935       -0.2124801627
 H         -1.6967976739       -0.2590733104       -1.6551757478
 H          1.9976894275        2.5746014602       -0.0131355030
 H         -2.2553337103       -1.5621910943        0.3759613685
 H          3.3296036963       -0.8005312819       -0.7375712274
  20
        -41.34878040
 O         -0.0657548310       -1.2361714125        0.2751975898
 O         -1.1713153229        0.2220849553       -1.8108552566
 O          0.8271523988        2.2102826978        0.5901612397
 O         -3.3079610388        0.4029461327        1.1653097904
 O          3.1294588066        0.3207512292       -0.1683211257
 O          1.8244966899       -2.3338209129       -0.2941862683
 C         -0.4453884556        0.1124818452        0.5231727984
 C         -1.5257447971        0.5567689026       -0.5010702957
 C          0.8278270751        0.8916866071        0.3992779116
 C         -2.8838653929       -0.0478525787       -0.0942823123
 C          1.8184659532        0.0392939542        0.0615299114
 C          1.2541354676       -1.2986605510       -0.0173361891
 H         -0.8878796345        0.1784123646        1.5241381765
 H         -1.5736105517        1.6510077964       -0.4717472362
 H         -3.6184309608        0.1821681321       -0.8755578015
 H         -2.7813215304       -1.1334476828       -0.0095453501
 H         -1.1425916781       -0.7413806365       -1.8955803321
 H          1.7139472064        2.5753531416        0.4367070937
 H         -3.5422784564        1.3392634384        1.1110745703
 H          3.5861654794       -0.5042195367       -0.3915966984
  20
        -41.34784511
 O          0.1515255665       -1.4375799771        0.0040147462
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 O          0.5363712731        2.0474808665        0.7272629406
 O         -3.1023861860        0.9780276377        0.8559258764
 O          3.1226714620        0.5547816615        0.1142803669
 O          2.2271653246       -2.2245845366       -0.4103064848
 C         -0.4423028009       -0.1832961604        0.3294911837
 C         -1.4761094686        0.1902370689       -0.7620342935
 C          0.7170565130        0.7628211699        0.4259945692
 C         -2.3613117922        1.3490562026       -0.2753808601
 C          1.8491347357        0.0793430044        0.1490719823
 C          1.4924569939       -1.3043108366       -0.1198424659
 H         -0.9792497181       -0.2857125046        1.2806669175
 H         -0.9398170575        0.4837064291       -1.6769095216
 H         -1.7437173260        2.1983175915        0.0164208702
 H         -3.0250805693        1.6403505157       -1.0987817396
 H         -1.8012465783       -1.6962654030       -1.1409777066
 H          1.3899599938        2.5118090592        0.7541102322
 H         -3.6148821955        0.1898461384        0.6225689371
 H          3.7210743111       -0.1770297074       -0.0989198056
  20
        -41.34768697
 O         -0.0833805604       -0.9359352562        1.2286403179
 O         -2.1565173079        1.9966328871        0.5875702637
 O          0.7748915395        2.1201133275       -0.4239198284
 O         -1.5345581020       -1.1379734539       -1.2304997399
 O          2.5924439259       -0.2786764445       -1.0576968578
 O          1.4227454153       -2.4729088610        0.5421416947
 C         -0.3436201507        0.4383115306        0.9827312277
 C         -1.7837661724        0.6583484914        0.4410382879
 C          0.7333130197        0.8605028727        0.0262745415
 C         -1.9334138216        0.1936164661       -1.0218214478
 C          1.5082993779       -0.2081059141       -0.2427992712
 C          0.9890638320       -1.3414553171        0.5138330350
 H         -0.2740510938        0.9777605505        1.9362557445
 H         -2.4579184848        0.0805344566        1.0836905027
 H         -2.9772209529        0.3511719194       -1.3227249826
 H         -1.2932636408        0.8000807485       -1.6680689854
 H         -1.5518245397        2.5558896142        0.0759257126
 H          1.5361450920        2.2422249701       -1.0138861940
 H         -2.1291701373       -1.7300889671       -0.7522164597
 H          2.9177464132       -1.1915443076       -1.0562629249
  20
        -41.34767928
 O          0.0544065936       -0.9667703694        1.3893442596
 O         -2.2410870757        1.7235925516        0.4655738216
 O          0.5835433497        2.0228370376       -0.4982918400
 O         -1.3242615004        0.1763038728       -1.7187390216
 O          2.4977737324       -0.2850168054       -1.1423179462
 O          1.5945001159       -2.4540159979        0.6597989139
 C         -0.3321210543        0.3601550200        1.0675563765
 C         -1.8205297622        0.4041781815        0.6126961451
 C          0.6383328221        0.7939823146        0.0086785506
 C         -2.0440669060       -0.4203959867       -0.6624895650
 C          1.4715984152       -0.2377486034       -0.2481630555
 C          1.0948472253       -1.3480759051        0.6076519069
 H         -0.2502049161        0.9815576334        1.9671252648
 H         -2.4192570622       -0.0314529661        1.4191111869
 H         -1.7108100019       -1.4512245153       -0.4849825534
 H         -3.1192363181       -0.4213900815       -0.8767017646
 H         -1.7716320288        2.1107974791       -0.2911029603
 H          1.2512175932        2.1310124218       -1.1948845707
 H         -1.5511876045       -0.2602323290       -2.5487073252
 H          2.9006846308       -1.1649985535       -1.0981093987
  20
        -41.34755988
 O          0.0755392676       -0.9699609569        1.4029510022
 O         -2.2106649731        1.7342368648        0.4820844940
 O          0.5548461405        2.0042840518       -0.5254751003
 O         -1.4031116043        0.1511084806       -1.7770518070
 O          2.4841142063       -0.2971835671       -1.1634911894
 O          1.6161529402       -2.4538715318        0.6710270304
 C         -0.3167984108        0.3545470136        1.0804695328
 C         -1.8017920480        0.4051202250        0.6308588682
 C          0.6361173898        0.7795906678        0.0008885076
 C         -2.0234923739       -0.4214269205       -0.6533154395
 C          1.4703471771       -0.2477800019       -0.2599772305
 C          1.1084417151       -1.3532926124        0.6133573554
 H         -0.2187666615        0.9769860690        1.9783586919
 H         -2.4118136614       -0.0276013216        1.4298580911
 H         -1.5857691403       -1.4129724662       -0.5182290724
 H         -3.1012088617       -0.5316690856       -0.8153309590
 H         -1.5613558921        2.2031687287       -0.0677406768
 H          1.2224546384        2.1157762128       -1.2214264788
 H         -1.8998234339        0.9413513755       -2.0326369020
 H          2.8760290577       -1.1828856225       -1.1381796864
  20
        -41.34740748
 O          0.1769160418       -1.0303203566        1.2515386314
 O         -2.1170309863        1.7149595468        0.4697730020
 O          0.7226778825        2.0823173480       -0.4253373243
 O         -3.1334925181       -0.3403648017       -1.3257974409
 O          2.7742813481       -0.1196355792       -1.0402958031
 O          1.8296300560       -2.4145463477        0.5700183997
 C         -0.2151350412        0.3111286480        1.0049321441
 C         -1.6651467055        0.3904388669        0.4623090242
 C          0.8113789538        0.8320873917        0.0404526543
 C         -1.7893464929       -0.2340445128       -0.9373793113
 C          1.6928438460       -0.1541849322       -0.2203576145
 C          1.2899954019       -1.3297091373        0.5432261953
 H         -0.1916350426        0.8595374591        1.9553164193
 H         -2.3164284071       -0.1597980376        1.1500269643
 H         -1.2302071828        0.3736483633       -1.6643192385
 H         -1.3844892690       -1.2483029597       -0.9312502295
 H         -1.4983280102        2.2684567067       -0.0333223090
 H          1.4704350363        2.2817641967       -1.0121729854
 H         -3.5634968914        0.5118126284       -1.1588007005
 H          3.2135967069       -0.9831499866       -1.0108193391
  20
        -41.34740649
 O         -0.0804883530       -1.2186729541        0.2979901378
 O         -1.1486122408        0.3114904302       -1.7992510818
 O          0.8434541583        2.2232872964        0.5691916068
 O         -3.2488789837        0.3602121303        1.1565171459
 O          3.1213179141        0.3103325366       -0.1923312897
 O          1.7953941457       -2.3362064257       -0.2796654656
 C         -0.4485529616        0.1357630173        0.5351408383
 C         -1.5334508187        0.5771895943       -0.4828558009
 C          0.8286714381        0.9045437480        0.3899982864
 C         -2.8668466545       -0.0905489583       -0.1219171703
 C          1.8099217409        0.0405792241        0.0521709444
 C          1.2361843213       -1.2933638004       -0.0072598045
 H         -0.8834498740        0.2224643624        1.5367102197
 H         -1.6357826927        1.6634620262       -0.3983943333
 H         -3.6075426762        0.1896755610       -0.8814467236
 H         -2.7322760280       -1.1815048755       -0.1297855456
 H         -1.0832261374       -0.6461612525       -1.9237407489
 H          1.7314234004        2.5787729733        0.3993865052
 H         -4.1042270228       -0.0247872727        1.3860373885
 H          3.5695438545       -0.5202146502       -0.4121331204
  20
        -41.34699823
 O          0.0619875615       -1.1355023352        0.6841433400
 O         -1.2908271829        0.1175461661       -1.4330881049
 O          0.9613887453        2.3046920850        0.3334979955
 O         -3.9110187462        0.2512870049       -0.2989802978
 O          3.1259729548        0.2911300238       -0.5058991016
 O          1.8430010236       -2.3341918203       -0.0202935222
 C         -0.2860698646        0.2402650848        0.7862543494
 C         -1.5097952685        0.5431940704       -0.1141594141
 C          0.9443841383        0.9743313823        0.3385369702
 C         -2.7822958592       -0.1021765147        0.4574597417
 C          1.8742174247        0.0659680393       -0.0261455813
 C          1.3191063404       -1.2609743671        0.1905067036
 H         -0.5424508609        0.4632829523        1.8264729457
 H         -1.6331311885        1.6294592143       -0.1677160542
 H         -2.6503166422       -1.1944160923        0.4772185305
 H         -2.9679848300        0.2515772091        1.4725316852
 H         -1.1679801631       -0.8432080635       -1.4360053161
 H          1.8043440890        2.6338065226       -0.0197582307
 H         -3.6930606000        0.1506845826       -1.2374779940
 H          3.5509340566       -0.5631974751       -0.6753801923
  20
        -41.34685668
 O          0.2851577297       -1.4015618023        0.5980011029
 O         -2.4411100844       -1.1066992375        0.1491201154
 O          0.6064500895        2.1512855612        0.2928335871
 O         -3.3712378180        1.3002183524       -1.0358733929
 O          3.0801356214        0.5501978193       -0.4902730036
 O          2.2656920325       -2.2684320699       -0.0544265763
 C         -0.3062215372       -0.1068811837        0.6922524625
 C         -1.5558991842       -0.0804792148       -0.2207476194
 C          0.7906111803        0.8317587040        0.2850794074
 C         -2.3301109462        1.2401809318       -0.0986201317
 C          1.8749679964        0.1005276112       -0.0527706430
 C          1.5547929108       -1.3059271317        0.1396595382
 H         -0.6276903016        0.0472473507        1.7284509770
 H         -1.2288055710       -0.2162713209       -1.2637345681
 H         -2.7206775025        1.3187729533        0.9241168441
 H         -1.6759560549        2.0868658615       -0.3047024155
 H         -1.9689526815       -1.9500861149        0.1616892693
 H          1.4144282475        2.6067003537        0.0010865935
 H         -3.9370049028        0.5264071663       -0.9035365669
 H          3.6614752563       -0.2111141207       -0.6379065401
  20
        -41.34671196
 O          0.1677462378       -1.5645494002        0.1867200609
 O         -2.6792034609       -1.0231092657       -0.3480166251
 O          0.4195001671        2.0002509644        0.6148053051
 O         -2.3043701540        1.8551916497        0.5270786926
 O          3.0062499489        0.5872568186       -0.1000794471
 O          2.2638128256       -2.2443508496       -0.3062023646
 C         -0.4919596094       -0.3208660646        0.4080720119
 C         -1.6068177112       -0.1735525757       -0.6641416960
 C          0.6045754976        0.7099877278        0.3625355223
 C         -2.1564553068        1.2605984800       -0.7414738405
 C          1.7606243438        0.0721477729        0.0605758524
 C          1.4831781958       -1.3495032765       -0.0543384429
 H         -0.9661692477       -0.3635101112        1.3964748791
 H         -1.1742660473       -0.4396716877       -1.6392498019
 H         -1.4665876918        1.8911771854       -1.3041941348
 H         -3.1131580014        1.2176823077       -1.2707149366
 H         -2.3736835763       -1.9392817393       -0.3276829382
 H         -0.5292134032        2.1852734941        0.8060369864
 H         -3.0131866790        1.4035327166        1.0046863927
 H          3.6150181877       -0.1445105883       -0.2889121327
  20
        -41.34667915
 O          0.0438338922       -1.0736132028        0.8195290061
 O         -1.1738295266        0.2646212770       -1.3597565334
 O          1.0128840455        2.3252113096        0.2881018313
 O         -3.7759905132       -0.0401033561       -0.4881095858
 O          3.0503168626        0.2117315296       -0.6398751769
 O          1.7306722286       -2.3474252599        0.0220688718
 C         -0.2711487911        0.3105106979        0.8614008253
 C         -1.4918613339        0.6125679317       -0.0352857585
 C          0.9559850177        0.9945058736        0.3308388760
 C         -2.7298158991       -0.1640268440        0.4430480043
 C          1.8261197412        0.0470484789       -0.0721019739
 C          1.2452643363       -1.2549068467        0.2269652594
 H         -0.5007634878        0.5865986691        1.8949011808
 H         -1.6715729960        1.6986797265        0.0226009547
 H         -2.4829354041       -1.2275621536        0.4866135910
 H         -3.0277281225        0.1768029659        1.4396060417
 H         -1.9848734623       -0.0741750359       -1.7738182225
 H          1.8310131204        2.6135566946       -0.1488745126
 H         -4.1342554280        0.8575808059       -0.4553255082
 H          3.4235853490       -0.6643685607       -0.8206353088
  20
        -41.34635424
 O          0.1566420657       -1.5346167456        0.2625975601
 O         -2.4333450938       -1.3296310640       -0.4691441982
 O          0.4618117749        2.0456388615        0.4860524836
 O         -2.1251514794        2.2633216336       -0.1404090812
 O          3.0134957732        0.5675132991       -0.1647833107
 O          2.2345437343       -2.2646928451       -0.2307943718
 C         -0.4771889341       -0.2800077895        0.5057345707
 C         -1.6833526404       -0.1454927860       -0.4618481496
 C          0.6246261910        0.7382691645        0.3404135575
 C         -2.6555252763        0.9653992365       -0.0245352475
 C          1.7697769614        0.0710686134        0.0581343239
 C          1.4743473136       -1.3487869718        0.0059830850
 H         -0.8534676437       -0.2974398722        1.5369169281
 H         -1.2917196736        0.0647743892       -1.4717580415
 H         -3.5688735203        0.8486042032       -0.6139938552
 H         -2.9094804334        0.8210054180        1.0279005694
 H         -1.8335730061       -2.0866328466       -0.5325984610
 H         -0.5009014715        2.2895474699        0.4614886448
 H         -2.1307879359        2.5403828701       -1.0668999715
 H          3.6147025785       -0.1779400759       -0.3210216635
  20
        -41.34621565
 O          0.0466417466       -1.1008292944        0.7331909821
 O         -1.2602439913        0.1960243628       -1.4209179833
 O          1.0029627184        2.3171143022        0.3194627605
 O         -3.8541542787        0.0078407466       -0.3917988020
 O          3.1044827652        0.2475400246       -0.5557658830
 O          1.7834634596       -2.3422997419       -0.0065870424
 C         -0.2774101741        0.2812110693        0.8093065592
 C         -1.4996207716        0.5942852633       -0.1012462589
 C          0.9593657693        0.9860499809        0.3365470618
 C         -2.7599775093       -0.0683778086        0.4825889100
 C          1.8595103510        0.0557032069       -0.0441276201
 C          1.2824679336       -1.2573252198        0.2022356117
 H         -0.5295044324        0.5274210277        1.8452674119
 H         -1.6074864300        1.6847298747       -0.1319045314
 H         -2.5675452251       -1.1348237473        0.6303008989
 H         -2.9928901892        0.3873608551        1.4535007248
 H         -1.4626584229       -0.7453638479       -1.5128616533
 H          1.8400702370        2.6243131840       -0.0655717138
 H         -4.0557894255        0.9347389270       -0.5775733586
 H          3.5008364260       -0.6190014160       -0.7326623629
  20
        -41.34600186
 O          0.1424903027       -1.4542742355        0.0232482825
 O         -2.2856308155       -0.8946650078       -1.1067694468
 O          0.5323116754        2.0326205313        0.7474978076
 O         -3.0336368354        0.9242872419        0.8723594332
 O          3.1099496328        0.5422421443        0.0807504974
 O          2.2183274159       -2.2331865990       -0.4162990613
 C         -0.4511065686       -0.1996328096        0.3344336394
 C         -1.4811472233        0.1921538436       -0.7680392295
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        -41.34586856
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        -41.34578742
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        -41.34576215
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        -41.34575986
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        -41.34565336
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        -41.34565094
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        -41.34561046
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        -41.34559915
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        -41.34548972
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        -41.34544647
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        -41.34536646
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        -41.34529092
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        -41.34519953
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        -41.34516936
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        -41.34498571
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        -41.34492705
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        -41.34488720
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        -41.34475678
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        -41.34474154
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        -41.34465516
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        -41.34463508
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        -41.34458354
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        -41.34455719
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        -41.34455021
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        -41.34450398
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        -41.34449236
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        -41.34446151
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        -41.34437107
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        -41.34436166
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        -41.34432842
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        -41.34424787
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        -41.34416458
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        -41.34408221
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        -41.34401815
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        -41.34399978
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        -41.34397760
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        -41.34389932
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        -41.34389115
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        -41.34385464
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        -41.34382417
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        -41.34372842
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        -41.34345275
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        -41.34343395
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        -41.34341315
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        -41.34340367
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        -41.34331044
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        -41.34328716
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        -41.34326158
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        -41.34321278
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        -41.34320129
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        -41.34318309
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        -41.34313844
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        -41.34309525
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        -41.34307067
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        -41.34306840
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        -41.34300625
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        -41.34297903
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        -41.34291719
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        -41.34290075
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        -41.34289871
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        -41.34279715
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        -41.34259812
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        -41.34253094
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        -41.34251000
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        -41.34249406
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        -41.34246813
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        -41.34241369
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        -41.34240775
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        -41.34236993
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        -41.34226278
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        -41.34223303
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        -41.34223215
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        -41.34217506
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        -41.34216903
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        -41.34213533
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        -41.34210627
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        -41.34208971
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        -41.34207704
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        -41.34199250
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        -41.34197000
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        -41.34195447
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        -41.34190984
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        -41.34186814
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        -41.34183215
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        -41.34167523
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        -41.34160473
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        -41.34155925
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        -41.34157329
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        -41.34148673
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        -41.34139900
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        -41.34139305
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        -41.34131508
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        -41.34129997
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        -41.34122489
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        -41.34108043
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        -41.34106580
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        -41.34098949
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        -41.34095830
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        -41.34082169
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        -41.34073673
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        -41.34068604
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        -41.34066666
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        -41.34065220
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        -41.34061264
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  20
        -41.34036626
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        -41.34021982
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        -41.33996717
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        -41.33992248
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        -41.33983507
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        -41.33979528
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        -41.33977289
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        -41.33975444
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        -41.33971319
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        -41.33953923
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        -41.33949400
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        -41.33941040
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        -41.33939457
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  20
        -41.33934818
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 H          3.3518181776        1.1989268922       -0.2552505798
                                                                                
                                                                                
      โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘โ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘โ–‘โ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ•—โ–‘โ–‘โ–‘โ–‘โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—      
      โ–ˆโ–ˆโ•”โ•โ•โ•โ•โ•โ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•”โ•โ•โ•โ•โ•โ–ˆโ–ˆโ•”โ•โ•โ•โ•โ•โ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–‘โ–‘โ–‘โ–ˆโ–ˆโ•”โ•โ•โ•โ•โ•      
      โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘โ–ˆโ–ˆโ•”โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ•šโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘โ–ˆโ–ˆโ•”โ–ˆโ–ˆโ–ˆโ–ˆโ•”โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•ฆโ•โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–‘โ–‘โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘      
      โ–ˆโ–ˆโ•”โ•โ•โ•โ–‘โ–‘โ–ˆโ–ˆโ•‘โ•šโ–ˆโ–ˆโ–ˆโ–ˆโ•‘โ–‘โ•šโ•โ•โ•โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•”โ•โ•โ•โ–‘โ–‘โ–ˆโ–ˆโ•‘โ•šโ–ˆโ–ˆโ•”โ•โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–‘โ–‘โ–‘โ–ˆโ–ˆโ•”โ•โ•โ•โ–‘โ–‘      
      โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ–‘โ•šโ–ˆโ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•”โ•โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ–‘โ•šโ•โ•โ–‘โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•ฆโ•โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—      
      โ•šโ•โ•โ•โ•โ•โ•โ•โ•šโ•โ•โ–‘โ–‘โ•šโ•โ•โ•โ•šโ•โ•โ•โ•โ•โ•โ–‘โ•šโ•โ•โ•โ•โ•โ•โ•โ•šโ•โ•โ–‘โ–‘โ–‘โ–‘โ–‘โ•šโ•โ•โ•šโ•โ•โ•โ•โ•โ•โ–‘โ•šโ•โ•โ•โ•โ•โ•โ•โ•šโ•โ•โ•โ•โ•โ•โ•      
                                                                                
       โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘โ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ•—โ–‘โ–‘โ–‘โ–‘โ–‘โ–ˆโ–ˆโ•—โ–‘โ–‘โ–‘โ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘       
       โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–‘โ–‘โ–‘โ•šโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ•”โ•โ–ˆโ–ˆโ•”โ•โ•โ•โ•โ•โ–ˆโ–ˆโ•”โ•โ•โ•โ•โ•โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•—       
       โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•”โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–‘โ–‘โ–‘โ–‘โ•šโ–ˆโ–ˆโ–ˆโ–ˆโ•”โ•โ–‘โ•šโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•”โ•       
       โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•‘โ•šโ–ˆโ–ˆโ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–‘โ–‘โ–‘โ–‘โ–‘โ•šโ–ˆโ–ˆโ•”โ•โ–‘โ–‘โ–‘โ•šโ•โ•โ•โ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•”โ•โ•โ•โ–‘โ–‘โ–ˆโ–ˆโ•”โ•โ•โ–ˆโ–ˆโ•—       
       โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•‘โ–‘โ•šโ–ˆโ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–ˆโ–ˆโ•‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–‘โ–‘โ–‘โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–‘โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•”โ•โ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ–ˆโ•—โ–ˆโ–ˆโ•‘โ–‘โ–‘โ–ˆโ–ˆโ•‘       
       โ•šโ•โ•โ–‘โ–‘โ•šโ•โ•โ•šโ•โ•โ–‘โ–‘โ•šโ•โ•โ•โ•šโ•โ•โ–‘โ–‘โ•šโ•โ•โ•šโ•โ•โ•โ•โ•โ•โ•โ–‘โ–‘โ–‘โ•šโ•โ•โ–‘โ–‘โ–‘โ•šโ•โ•โ•โ•โ•โ•โ–‘โ•šโ•โ•โ•โ•โ•โ•โ•โ•šโ•โ•โ–‘โ–‘โ•šโ•โ•       
                                                                                
                          ---   Andrea Pellegrini  ---                          
                                                                                
                                                                                
                                  MIT license                                   
                                    v. 1.0.0                                    
                                                                                
          GitHub: https://github.com/andre-cloud/Ensemble_Analyser.git          
                                                                                

======================================================================
                          Calculation Input                           
======================================================================
Ensemble: 134 confromer(s)
Protocols: 3
Temperature: 298.15 K
CPU cores: 44

Protocol Steps:
000. โ†’ ExtOpt/def2-mTZVP [SMD(methanol)] | SP 
 	thrG    : 0.25 kcal/mol
	thrB    : 1e-05 cm-1
	thrGMAX : 6 kcal/mol

001. โ†’ r2SCAN-3c/def2-mTZVPP [SMD(methanol)] | OPT+FREQ 
 	thrG    : 0.5 kcal/mol
	thrB    : 5e-05 cm-1
	thrGMAX : 3.5 kcal/mol

002. โ†’ wB97X-D4rev/def2-QZVPP [SMD(methanol)] | SP 
 	thrG    : 0.25 kcal/mol
	thrB    : 1e-05 cm-1
	thrGMAX : 6 kcal/mol

======================================================================

PCA Analysis:
  Conformers: 134
  Include H: False
  Output: initial_pca.png
Starting PCA analysis on 134 conformers
  Hydrogen inclusion: False
  Requested clusters: auto-detect
  Set cluster attribute: False
  Optimal clusters detected: 19
โœ“ PCA completed: 19 clusters identified, variance explained: 86.4% (PC1+PC2)

======================================================================
                           PROTOCOL 0 - SP                            
======================================================================
Level: ExtOpt/def2-mTZVP
Active conformers: 134
======================================================================
001. โ†’ CONF 001 | Protocol 0
	B not found, storing a versor
	M not found, storing a versor
โŠ— No previous thermochemical data found for conformer 1: setting G, H, S, ZPVE to NaN.
	โœ“ E = -684.88136249 Eh | Time: 1.0s
002. โ†’ CONF 002 | Protocol 0
	B not found, storing a versor
	M not found, storing a versor
โŠ— No previous thermochemical data found for conformer 2: setting G, H, S, ZPVE to NaN.
	โœ“ E = -684.87708379 Eh | Time: 1.1s

[...]

Total elapsed time for protocol 0: 0:02:17.065074

**********************************************************************
                        Summary Before Pruning                        
**********************************************************************

Conformers    E [Eh]           G-E [Eh]    G [Eh]    B [cm-1]    โˆ†G [kcal/mol]    Pop [%]    Elap. time [sec]    # Cluster
------------  ---------------  ----------  --------  ----------  ---------------  ---------  ------------------  -----------
1             -684.8813624869  nan         nan       1.00000     nan              nan        1.01
2             -684.8770837881  nan         nan       1.00000     nan              nan        1.09
[...]


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
       Pruning sequence       
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Filtering conformers above 6 kcal/mol energy window

******************************
Conformers over energy window 
******************************

          โˆ†E [kcal/mol]
--------  ---------------
Conf 38   8.38
Conf 50   7.29
Conf 56   6.82
Conf 57   6.43
Conf 61   9.04
Conf 62   6.72
Conf 63   7.47
Conf 65   7.34
Conf 66   7.85
Conf 67   6.68
Conf 69   6.81
Conf 74   7.97
Conf 75   6.47
Conf 76   6.08
Conf 77   6.30
Conf 78   6.30
Conf 81   9.39
Conf 82   9.76
Conf 83   8.16
Conf 84   6.50
Conf 85   6.10
Conf 86   6.04
Conf 88   7.71
Conf 90   7.54
Conf 91   9.02
Conf 92   7.36
Conf 93   6.52
Conf 94   6.74
Conf 95   8.63
Conf 96   8.66
Conf 97   9.25
Conf 98   8.28
Conf 99   6.50
Conf 100  9.42
Conf 101  8.40
Conf 102  8.54
Conf 103  8.62
Conf 105  8.79
Conf 106  7.96
Conf 107  6.55
Conf 108  6.97
Conf 109  7.29
Conf 110  8.23
Conf 111  7.80
Conf 113  9.82
Conf 114  6.47
Conf 115  8.25
Conf 116  9.75
Conf 117  9.50
Conf 118  7.98
Conf 119  6.72
Conf 120  9.45
Conf 121  9.50
Conf 122  9.85
Conf 123  8.62
Conf 124  8.18
Conf 125  9.43
Conf 126  8.57
Conf 127  9.26
Conf 128  9.71
Conf 129  7.10
Conf 130  9.45
Conf 131  10.36
Conf 132  10.46
Conf 133  9.55
Conf 134  10.01
โœ“ Deactivated 66 conformer(s)

No conformers deactivated by similarity check

โœ“ Pruning completed in 0.0037s
Initial: 134 โ†’ Final: 68 (50.7% retained)
Deactivated: 66


**********************************************************************
                        Summary After Pruning                         
**********************************************************************

Conformers    E [Eh]           G-E [Eh]    G [Eh]    B [cm-1]    โˆ†G [kcal/mol]    Pop [%]    Elap. time [sec]    # Cluster
------------  ---------------  ----------  --------  ----------  ---------------  ---------  ------------------  -----------
1             -684.8813624869  nan         nan       1.00000     0.00             63.10      1.01
3             -684.8801147932  nan         nan       1.00000     0.78             16.82      0.99
9             -684.8786320879  nan         nan       1.00000     1.71             3.50       0.95
10            -684.8784951996  nan         nan       1.00000     1.80             3.02       0.96
6             -684.8779888364  nan         nan       1.00000     2.12             1.77       0.97
5             -684.8778709591  nan         nan       1.00000     2.19             1.56       0.98
8             -684.8776561701  nan         nan       1.00000     2.33             1.24       0.98
18            -684.8776291368  nan         nan       1.00000     2.34             1.21       0.91
7             -684.8773705809  nan         nan       1.00000     2.50             0.92       0.97
30            -684.877353162   nan         nan       1.00000     2.52             0.90       0.97
21            -684.8772958041  nan         nan       1.00000     2.55             0.85       0.97
11            -684.8772424167  nan         nan       1.00000     2.59             0.80       0.93
2             -684.8770837881  nan         nan       1.00000     2.68             0.68       1.09
54            -684.8766229554  nan         nan       1.00000     2.97             0.42       0.98
12            -684.8761584122  nan         nan       1.00000     3.27             0.25       0.99
16            -684.8761331853  nan         nan       1.00000     3.28             0.25       0.94
29            -684.8760935203  nan         nan       1.00000     3.31             0.24       0.95
36            -684.8759486125  nan         nan       1.00000     3.40             0.20       0.96
55            -684.8758794597  nan         nan       1.00000     3.44             0.19       1.05
23            -684.875857198   nan         nan       1.00000     3.45             0.18       0.97
19            -684.8757034298  nan         nan       1.00000     3.55             0.16       0.99
14            -684.8756677752  nan         nan       1.00000     3.57             0.15       0.95
37            -684.8755803194  nan         nan       1.00000     3.63             0.14       0.93
4             -684.8755472361  nan         nan       1.00000     3.65             0.13       1.01
13            -684.8754584938  nan         nan       1.00000     3.70             0.12       0.94
17            -684.8754445052  nan         nan       1.00000     3.71             0.12       0.96
28            -684.875240039   nan         nan       1.00000     3.84             0.10       0.96
48            -684.8751239704  nan         nan       1.00000     3.91             0.08       0.98
43            -684.8749326029  nan         nan       1.00000     4.03             0.07       0.92
22            -684.8748224558  nan         nan       1.00000     4.10             0.06       1.02
41            -684.8748096281  nan         nan       1.00000     4.11             0.06       0.93
73            -684.8747476662  nan         nan       1.00000     4.15             0.06       1.01
20            -684.8746456469  nan         nan       1.00000     4.21             0.05       0.94
47            -684.8745476573  nan         nan       1.00000     4.28             0.05       0.97
45            -684.8745473843  nan         nan       1.00000     4.28             0.05       0.93
52            -684.8744997344  nan         nan       1.00000     4.31             0.04       0.94
59            -684.8744576705  nan         nan       1.00000     4.33             0.04       0.96
42            -684.8744446752  nan         nan       1.00000     4.34             0.04       0.92
40            -684.8744252157  nan         nan       1.00000     4.35             0.04       0.91
27            -684.8741568245  nan         nan       1.00000     4.52             0.03       0.94
72            -684.8741467205  nan         nan       1.00000     4.53             0.03       0.98
25            -684.8741053367  nan         nan       1.00000     4.55             0.03       0.90
15            -684.8740923953  nan         nan       1.00000     4.56             0.03       0.96
31            -684.8740922126  nan         nan       1.00000     4.56             0.03       0.96
46            -684.8738835078  nan         nan       1.00000     4.69             0.02       0.96
49            -684.8737191008  nan         nan       1.00000     4.80             0.02       0.97
32            -684.8736895812  nan         nan       1.00000     4.81             0.02       0.94
51            -684.873601002   nan         nan       1.00000     4.87             0.02       1.00
44            -684.8735897212  nan         nan       1.00000     4.88             0.02       0.98
64            -684.8734908016  nan         nan       1.00000     4.94             0.02       1.11
53            -684.8730632191  nan         nan       1.00000     5.21             0.01       0.96
60            -684.8730356901  nan         nan       1.00000     5.23             0.01       0.93
39            -684.8727577909  nan         nan       1.00000     5.40             0.01       0.92
35            -684.8726445736  nan         nan       1.00000     5.47             0.01       0.94
71            -684.8725122891  nan         nan       1.00000     5.55             0.01       0.95
89            -684.8724636314  nan         nan       1.00000     5.58             0.01       0.96
104           -684.8724513948  nan         nan       1.00000     5.59             0.01       0.91
34            -684.8724454869  nan         nan       1.00000     5.60             0.00       0.96
70            -684.8724229543  nan         nan       1.00000     5.61             0.00       0.92
80            -684.8723263494  nan         nan       1.00000     5.67             0.00       0.98
24            -684.8722806296  nan         nan       1.00000     5.70             0.00       1.00
58            -684.8722561722  nan         nan       1.00000     5.71             0.00       0.99
87            -684.8721230461  nan         nan       1.00000     5.80             0.00       0.91
68            -684.8721064275  nan         nan       1.00000     5.81             0.00       0.96
26            -684.8720540812  nan         nan       1.00000     5.84             0.00       1.06
79            -684.8719816267  nan         nan       1.00000     5.89             0.00       0.98
112           -684.8719656358  nan         nan       1.00000     5.90             0.00       0.95
33            -684.8718746485  nan         nan       1.00000     5.95             0.00       1.01

**************************************************
    Energetic Summary of the active conformers    
**************************************************

Conformer    โˆ†E [Eh]          Boltzamnn Pop. on โˆ†E    โˆ†(E+ZPVE) [Eh]    Boltzamnn Pop. on โˆ†(E+ZPVE)    โˆ†H [Eh]    Boltzamnn Pop. on โˆ†H    โˆ†G [Eh]    Boltzamnn Pop. on โˆ†G
-----------  ---------------  ----------------------  ----------------  -----------------------------  ---------  ----------------------  ---------  ----------------------
Conf 1       -684.8813624869  63.10                   nan               nan                            nan        nan                     nan        nan
Conf 3       -684.8801147932  16.82                   nan               nan                            nan        nan                     nan        nan
Conf 9       -684.8786320879  3.50                    nan               nan                            nan        nan                     nan        nan
Conf 10      -684.8784951996  3.02                    nan               nan                            nan        nan                     nan        nan
Conf 6       -684.8779888364  1.77                    nan               nan                            nan        nan                     nan        nan
Conf 5       -684.8778709591  1.56                    nan               nan                            nan        nan                     nan        nan
Conf 8       -684.8776561701  1.24                    nan               nan                            nan        nan                     nan        nan
Conf 18      -684.8776291368  1.21                    nan               nan                            nan        nan                     nan        nan
Conf 7       -684.8773705809  0.92                    nan               nan                            nan        nan                     nan        nan
Conf 30      -684.877353162   0.90                    nan               nan                            nan        nan                     nan        nan
Conf 21      -684.8772958041  0.85                    nan               nan                            nan        nan                     nan        nan
Conf 11      -684.8772424167  0.80                    nan               nan                            nan        nan                     nan        nan
Conf 2       -684.8770837881  0.68                    nan               nan                            nan        nan                     nan        nan
Conf 54      -684.8766229554  0.42                    nan               nan                            nan        nan                     nan        nan
Conf 12      -684.8761584122  0.25                    nan               nan                            nan        nan                     nan        nan
Conf 16      -684.8761331853  0.25                    nan               nan                            nan        nan                     nan        nan
Conf 29      -684.8760935203  0.24                    nan               nan                            nan        nan                     nan        nan
Conf 36      -684.8759486125  0.20                    nan               nan                            nan        nan                     nan        nan
Conf 55      -684.8758794597  0.19                    nan               nan                            nan        nan                     nan        nan
Conf 23      -684.875857198   0.18                    nan               nan                            nan        nan                     nan        nan
Conf 19      -684.8757034298  0.16                    nan               nan                            nan        nan                     nan        nan
Conf 14      -684.8756677752  0.15                    nan               nan                            nan        nan                     nan        nan
Conf 37      -684.8755803194  0.14                    nan               nan                            nan        nan                     nan        nan
Conf 4       -684.8755472361  0.13                    nan               nan                            nan        nan                     nan        nan
Conf 13      -684.8754584938  0.12                    nan               nan                            nan        nan                     nan        nan
Conf 17      -684.8754445052  0.12                    nan               nan                            nan        nan                     nan        nan
Conf 28      -684.875240039   0.10                    nan               nan                            nan        nan                     nan        nan
Conf 48      -684.8751239704  0.08                    nan               nan                            nan        nan                     nan        nan
Conf 43      -684.8749326029  0.07                    nan               nan                            nan        nan                     nan        nan
Conf 22      -684.8748224558  0.06                    nan               nan                            nan        nan                     nan        nan
Conf 41      -684.8748096281  0.06                    nan               nan                            nan        nan                     nan        nan
Conf 73      -684.8747476662  0.06                    nan               nan                            nan        nan                     nan        nan
Conf 20      -684.8746456469  0.05                    nan               nan                            nan        nan                     nan        nan
Conf 47      -684.8745476573  0.05                    nan               nan                            nan        nan                     nan        nan
Conf 45      -684.8745473843  0.05                    nan               nan                            nan        nan                     nan        nan
Conf 52      -684.8744997344  0.04                    nan               nan                            nan        nan                     nan        nan
Conf 59      -684.8744576705  0.04                    nan               nan                            nan        nan                     nan        nan
Conf 42      -684.8744446752  0.04                    nan               nan                            nan        nan                     nan        nan
Conf 40      -684.8744252157  0.04                    nan               nan                            nan        nan                     nan        nan
Conf 27      -684.8741568245  0.03                    nan               nan                            nan        nan                     nan        nan
Conf 72      -684.8741467205  0.03                    nan               nan                            nan        nan                     nan        nan
Conf 25      -684.8741053367  0.03                    nan               nan                            nan        nan                     nan        nan
Conf 15      -684.8740923953  0.03                    nan               nan                            nan        nan                     nan        nan
Conf 31      -684.8740922126  0.03                    nan               nan                            nan        nan                     nan        nan
Conf 46      -684.8738835078  0.02                    nan               nan                            nan        nan                     nan        nan
Conf 49      -684.8737191008  0.02                    nan               nan                            nan        nan                     nan        nan
Conf 32      -684.8736895812  0.02                    nan               nan                            nan        nan                     nan        nan
Conf 51      -684.873601002   0.02                    nan               nan                            nan        nan                     nan        nan
Conf 44      -684.8735897212  0.02                    nan               nan                            nan        nan                     nan        nan
Conf 64      -684.8734908016  0.02                    nan               nan                            nan        nan                     nan        nan
Conf 53      -684.8730632191  0.01                    nan               nan                            nan        nan                     nan        nan
Conf 60      -684.8730356901  0.01                    nan               nan                            nan        nan                     nan        nan
Conf 39      -684.8727577909  0.01                    nan               nan                            nan        nan                     nan        nan
Conf 35      -684.8726445736  0.01                    nan               nan                            nan        nan                     nan        nan
Conf 71      -684.8725122891  0.01                    nan               nan                            nan        nan                     nan        nan
Conf 89      -684.8724636314  0.01                    nan               nan                            nan        nan                     nan        nan
Conf 104     -684.8724513948  0.01                    nan               nan                            nan        nan                     nan        nan
Conf 34      -684.8724454869  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 70      -684.8724229543  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 80      -684.8723263494  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 24      -684.8722806296  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 58      -684.8722561722  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 87      -684.8721230461  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 68      -684.8721064275  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 26      -684.8720540812  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 79      -684.8719816267  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 112     -684.8719656358  0.00                    nan               nan                            nan        nan                     nan        nan
Conf 33      -684.8718746485  0.00                    nan               nan                            nan        nan                     nan        nan

**************************************************
            Ensemble Average Energies             
**************************************************

T [K]    E_av [Eh]           E+ZPVE_av [Eh]    H_av [Eh]    G_av [Eh]
-------  ------------------  ----------------  -----------  -----------
298.15   -684.8803829333704  nan               nan          nan

----------------------------------------
          Spectra convolution           
----------------------------------------
โŠ— No calculation of IR graphs. Skipping
โŠ— No calculation of VCD graphs. Skipping
โŠ— No calculation of UV graphs. Skipping
โŠ— No calculation of ECD graphs. Skipping

โœ“ Sprectra convolution completed in 0.0859s


Protocol 0 completed in 0:02:17.169987
Active: 68 | Deactivated: 66

======================================================================
                            END PROTOCOL 0                            
======================================================================


======================================================================
                        PROTOCOL 1 - OPT+FREQ                         
======================================================================
Level: r2SCAN-3c/def2-mTZVPP
Active conformers: 68
======================================================================
001. โ†’ CONF 001 | Protocol 1
	โœ“ E = -684.73433702 Eh | Time: 178.6s | Imag. Freq 0 
002. โ†’ CONF 002 | Protocol 1
	โœ“ E = -684.73389342 Eh | Time: 157.4s | Imag. Freq 0 

[...]

Total elapsed time for protocol 1: 3:24:59.355608

**********************************************************************
                        Summary Before Pruning                        
**********************************************************************

Conformers    E [Eh]             G-E [Eh]             G [Eh]              B [cm-1]    โˆ†G [kcal/mol]    Pop [%]    Elap. time [sec]    # Cluster
------------  -----------------  -------------------  ------------------  ----------  ---------------  ---------  ------------------  -----------
1             -684.734337017943  0.11078618968633691  -684.6235508282566  0.05793     nan              nan        178.63
2             -684.733893422971  0.11068324091615978  -684.6232101820549  0.05789     nan              nan        157.39
[...]


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
       Pruning sequence       
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Filtering conformers above 3.5 kcal/mol energy window

******************************
Conformers over energy window 
******************************

         โˆ†E [kcal/mol]
-------  ---------------
Conf 28  3.64
Conf 32  3.60
Conf 34  3.50
Conf 79  5.19
โœ“ Deactivated 4 conformer(s)


******************************
Conformer pruned by โˆ†B and โˆ†E 
******************************

Check    Ref    โˆ†E [kcal/mol]    โˆ†B [cmโปยน]    โˆ†m [Debye]    ฮปi RMSD    Deactivate
-------  -----  ---------------  -----------  ------------  ---------  ------------
2        1      0.21             0.00         0.04          0.51       True
12       9      0.40             0.00         1.77          0.08       True
14       9      0.27             0.00         1.06          0.01       True
17       5      0.15             0.00         0.36          0.33       True
25       15     0.17             0.00         0.35          0.08       True
26       4      0.38             0.00         0.75          0.05       True
27       24     0.45             0.00         0.93          0.05       True
37       13     0.44             0.00         1.84          0.10       True
40       6      0.44             0.00         0.23          0.78       True
44       24     0.11             0.00         1.20          0.41       True
46       31     0.17             0.00         0.25          0.91       True
53       31     0.03             0.00         0.25          0.91       True
54       41     0.39             0.00         0.01          0.02       True
64       21     0.12             0.00         0.59          0.16       True
70       5      0.48             0.00         0.49          0.34       True
72       41     0.11             0.00         0.01          0.45       True
73       47     0.12             0.00         1.01          0.03       True
87       15     0.23             0.00         0.07          0.46       True
104      52     0.13             0.00         1.07          0.47       True

โœ“ Pruning completed in 0.0145s
Initial: 68 โ†’ Final: 45 (66.2% retained)
Deactivated: 23


**********************************************************************
                        Summary After Pruning                         
**********************************************************************

Conformers    E [Eh]             G-E [Eh]             G [Eh]              B [cm-1]    โˆ†G [kcal/mol]    Pop [%]    Elap. time [sec]    # Cluster
------------  -----------------  -------------------  ------------------  ----------  ---------------  ---------  ------------------  -----------
9             -684.734665340113  0.11044178749125422  -684.6242235526217  0.06062     0.00             18.01      171.14
36            -684.734315107605  0.11034965162559729  -684.6239654559794  0.06045     0.16             13.70      170.14
1             -684.734337017943  0.11078618968633691  -684.6235508282566  0.05793     0.42             8.83       178.63
21            -684.733061818697  0.11001511261474661  -684.6230467060823  0.06080     0.74             5.17       164.81
43            -684.732731253804  0.10996062930803419  -684.622770624496   0.05831     0.91             3.86       198.93
35            -684.733131077477  0.11042692433477441  -684.6227041531422  0.05780     0.95             3.60       173.94
8             -684.732844170182  0.11014181074847329  -684.6227023594336  0.05792     0.95             3.59       159.25
80            -684.732614716846  0.1102592158420066   -684.622355501004   0.05806     1.17             2.49       166.85
3             -684.7323405462    0.10999790498283346  -684.6223426412172  0.05984     1.18             2.45       181.39
49            -684.731903928484  0.10956784819984478  -684.6223360802842  0.05524     1.18             2.44       158.36
58            -684.732449816724  0.11011943635026     -684.6223303803738  0.05822     1.19             2.42       168.06
24            -684.73278632205   0.11046737228207348  -684.6223189497679  0.05812     1.20             2.39       175.92
13            -684.734282837475  0.11205136912985836  -684.6222314683451  0.05724     1.25             2.18       174.11
29            -684.733031441986  0.11084570170544339  -684.6221857402805  0.05746     1.28             2.08       184.01
19            -684.733471951317  0.1113123708872763   -684.6221595804298  0.05786     1.30             2.02       157.48
11            -684.733833428864  0.11168892862485791  -684.6221445002392  0.05911     1.30             1.99       166.62
52            -684.732744667148  0.11062874927210942  -684.6221159178759  0.05765     1.32             1.93       170.40
23            -684.732136115968  0.11007877128463406  -684.6220573446834  0.05647     1.36             1.81       167.02
7             -684.731798699216  0.10980090270390974  -684.6219977965121  0.05519     1.40             1.70       163.52
30            -684.731322234932  0.10947887334168627  -684.6218433615903  0.05642     1.49             1.45       188.82
22            -684.733882291767  0.11204399366101825  -684.621838298106   0.05704     1.50             1.44       175.53
10            -684.73167947606   0.10985834318285015  -684.6218211328771  0.05946     1.51             1.41       158.64
5             -684.731809382019  0.11015522379409504  -684.6216541582249  0.05797     1.61             1.18       223.45
16            -684.733348155037  0.11202636599045945  -684.6213217890465  0.05896     1.82             0.83       154.86
20            -684.731319120488  0.11004969141072252  -684.6212694290773  0.05496     1.85             0.79       183.67
6             -684.732290889698  0.11107219392408751  -684.6212186957739  0.05779     1.89             0.75       180.16
15            -684.731298265214  0.11023221327775445  -684.6210660519363  0.05989     1.98             0.63       190.29
4             -684.730268194067  0.11032647075694513  -684.61994172331    0.05627     2.69             0.19       249.93
31            -684.732438209736  0.11112331937908948  -684.6213148903569  0.05804     1.83             0.83       164.92
33            -684.730969762578  0.11035886185379695  -684.6206109007242  0.05943     2.27             0.39       149.31
18            -684.729908951754  0.11071269882756951  -684.6191962529265  0.05566     3.15             0.09       156.35
47            -684.731135334676  0.10973288083448551  -684.6214024538415  0.05554     1.77             0.91       181.32
48            -684.73168850708   0.11053230150719173  -684.6211562055728  0.05965     1.92             0.70       182.27
45            -684.731675821631  0.11063583374129848  -684.6210399878897  0.05639     2.00             0.62       164.32
59            -684.73125574206   0.1102244057151438   -684.6210313363448  0.05976     2.00             0.61       159.46
60            -684.732920527237  0.11200572215602733  -684.6209148050809  0.05918     2.08             0.54       177.01
55            -684.730853210096  0.1101439023674402   -684.6207093077286  0.05634     2.21             0.43       196.36
39            -684.731374148663  0.11113315060663354  -684.6202409980564  0.05958     2.50             0.26       185.55
42            -684.733441488013  0.11201324289334025  -684.6214282451197  0.05934     1.75             0.93       179.14
41            -684.732677862196  0.11173314480333829  -684.6209447173927  0.05768     2.06             0.56       211.57
51            -684.729863757107  0.11103599914235929  -684.6188277579646  0.05491     3.39             0.06       269.84
68            -684.73079978842   0.10991605524486658  -684.6208837331751  0.05509     2.10             0.52       169.77
89            -684.731044615311  0.10977002015158632  -684.6212745951594  0.05580     1.85             0.79       181.88
112           -684.731612155043  0.11120082323486713  -684.6204113318081  0.05756     2.39             0.32       206.89
71            -684.729323346729  0.11008852790030232  -684.6192348188287  0.05462     3.13             0.09       197.31

**************************************************
    Energetic Summary of the active conformers    
**************************************************

Conformer    โˆ†E [Eh]            Boltzamnn Pop. on โˆ†E    โˆ†(E+ZPVE) [Eh]      Boltzamnn Pop. on โˆ†(E+ZPVE)    โˆ†H [Eh]             Boltzamnn Pop. on โˆ†H    โˆ†G [Eh]             Boltzamnn Pop. on โˆ†G
-----------  -----------------  ----------------------  ------------------  -----------------------------  ------------------  ----------------------  ------------------  ----------------------
Conf 9       -684.734665340113  13.86                   -684.5862859816526  15.29                          -684.5739984921739  13.99                   -684.6242235526217  18.01
Conf 36      -684.734315107605  9.56                    -684.5860264202161  11.61                          -684.5736909085268  10.10                   -684.6239654559794  13.70
Conf 1       -684.734337017943  9.78                    -684.5858251384717  9.38                           -684.5736645251685  9.82                    -684.6235508282566  8.83
Conf 21      -684.733061818697  2.53                    -684.5849950020532  3.89                           -684.5724984658536  2.85                    -684.6230467060823  5.17
Conf 43      -684.732731253804  1.78                    -684.5847582521031  3.03                           -684.5722996075152  2.31                    -684.622770624496   3.86
Conf 35      -684.733131077477  2.73                    -684.5847794443166  3.10                           -684.5724944419223  2.84                    -684.6227041531422  3.60
Conf 8       -684.732844170182  2.01                    -684.5845832536565  2.52                           -684.572187588651   2.05                    -684.6227023594336  3.59
Conf 80      -684.732614716846  1.58                    -684.5843687223185  2.01                           -684.5720490315273  1.77                    -684.622355501004   2.49
Conf 3       -684.7323405462    1.18                    -684.5842557092503  1.78                           -684.5718825553777  1.49                    -684.6223426412172  2.45
Conf 49      -684.731903928484  0.74                    -684.5840912642207  1.49                           -684.571555589827   1.05                    -684.6223360802842  2.44
Conf 58      -684.732449816724  1.32                    -684.5843408311976  1.95                           -684.5719894340123  1.66                    -684.6223303803738  2.42
Conf 24      -684.73278632205   1.89                    -684.5844010859171  2.07                           -684.5720890582877  1.85                    -684.6223189497679  2.39
Conf 13      -684.734282837475  9.24                    -684.585363348252   5.75                           -684.5734825034122  8.10                    -684.6222314683451  2.18
Conf 29      -684.733031441986  2.45                    -684.5849053702023  3.54                           -684.5728759853193  4.26                    -684.6221857402805  2.08
Conf 19      -684.733471951317  3.91                    -684.5845773396845  2.50                           -684.5724106629473  2.60                    -684.6221595804298  2.02
Conf 11      -684.733833428864  5.74                    -684.5850820684119  4.27                           -684.5731036585762  5.42                    -684.6221445002392  1.99
Conf 52      -684.732744667148  1.81                    -684.5841470146656  1.59                           -684.5719492132737  1.60                    -684.6221159178759  1.93
Conf 23      -684.732136115968  0.95                    -684.5841606082828  1.61                           -684.571848349222   1.43                    -684.6220573446834  1.81
Conf 7       -684.731798699216  0.66                    -684.5838379996203  1.14                           -684.571449812029   0.94                    -684.6219977965121  1.70
Conf 30      -684.731322234932  0.40                    -684.5836846478508  0.97                           -684.5712330667764  0.75                    -684.6218433615903  1.45
Conf 22      -684.733882291767  6.04                    -684.5849307715072  3.64                           -684.573094128273   5.37                    -684.621838298106   1.44
Conf 10      -684.73167947606   0.59                    -684.583710210554   1.00                           -684.5712361775193  0.75                    -684.6218211328771  1.41
Conf 5       -684.731809382019  0.67                    -684.5837718398293  1.07                           -684.5712559822257  0.77                    -684.6216541582249  1.18
Conf 16      -684.733348155037  3.43                    -684.5843639202901  1.99                           -684.5724594464035  2.74                    -684.6213217890465  0.83
Conf 20      -684.731319120488  0.40                    -684.5831120370635  0.53                           -684.5707420236994  0.44                    -684.6212694290773  0.79
Conf 6       -684.732290889698  1.12                    -684.5836220216956  0.91                           -684.5713556379715  0.85                    -684.6212186957739  0.75
Conf 15      -684.731298265214  0.39                    -684.5831775926875  0.57                           -684.5707946654892  0.47                    -684.6210660519363  0.63
Conf 4       -684.730268194067  0.13                    -684.582085418691   0.18                           -684.5697294190503  0.15                    -684.61994172331    0.19
Conf 31      -684.732438209736  1.31                    -684.5836735675665  0.96                           -684.5714615765891  0.95                    -684.6213148903569  0.83
Conf 33      -684.730969762578  0.28                    -684.5827068640466  0.34                           -684.5703838829255  0.30                    -684.6206109007242  0.39
Conf 18      -684.729908951754  0.09                    -684.5816047500437  0.11                           -684.5693712183205  0.10                    -684.6191962529265  0.09
Conf 47      -684.731135334676  0.33                    -684.5832986813075  0.65                           -684.5707938424092  0.47                    -684.6214024538415  0.91
Conf 48      -684.73168850708   0.59                    -684.5833585392937  0.69                           -684.5711056252647  0.65                    -684.6211562055728  0.70
Conf 45      -684.731675821631  0.58                    -684.5833434845504  0.68                           -684.5711558316432  0.69                    -684.6210399878897  0.62
Conf 59      -684.73125574206   0.37                    -684.5831411522411  0.55                           -684.5707518951099  0.45                    -684.6210313363448  0.61
Conf 60      -684.732920527237  2.18                    -684.5839604182852  1.30                           -684.5720484072652  1.77                    -684.6209148050809  0.54
Conf 55      -684.730853210096  0.24                    -684.5827996068246  0.38                           -684.5704752544692  0.33                    -684.6207093077286  0.43
Conf 39      -684.731374148663  0.42                    -684.5825609359598  0.30                           -684.5704923495786  0.34                    -684.6202409980564  0.26
Conf 42      -684.733441488013  3.79                    -684.5844763443108  2.25                           -684.5725678312086  3.07                    -684.6214282451197  0.93
Conf 41      -684.732677862196  1.69                    -684.583780334509   1.07                           -684.5716709246207  1.19                    -684.6209447173927  0.56
Conf 51      -684.729863757107  0.09                    -684.5814144589004  0.09                           -684.5691211736447  0.08                    -684.6188277579646  0.06
Conf 68      -684.73079978842   0.23                    -684.5827443626143  0.36                           -684.5703430621174  0.29                    -684.6208837331751  0.52
Conf 89      -684.731044615311  0.30                    -684.5829698022989  0.46                           -684.5705997593151  0.38                    -684.6212745951594  0.79
Conf 112     -684.731612155043  0.55                    -684.5828023140278  0.38                           -684.5707210482288  0.43                    -684.6204113318081  0.32
Conf 71      -684.729323346729  0.05                    -684.5813003620303  0.08                           -684.568922392965   0.06                    -684.6192348188287  0.09

**************************************************
            Ensemble Average Energies             
**************************************************

T [K]    E_av [Eh]           E+ZPVE_av [Eh]      H_av [Eh]           G_av [Eh]
-------  ------------------  ------------------  ------------------  ------------------
298.15   -684.7335644931289  -684.5850282851541  -684.5728510119255  -684.6228770508599

----------------------------------------
          Spectra convolution           
----------------------------------------

-----------------------------------
      IR Spectra convolution       
-----------------------------------
Using default parameters, Reference IR Spectra not found
Shift: 1	FWHM: 10

-----------------------------------
      VCD Spectra convolution      
-----------------------------------
Using default parameters, Reference VCD Spectra not found
Shift: 1	FWHM: 10
โŠ— No calculation of UV graphs. Skipping
โŠ— No calculation of ECD graphs. Skipping

โœ“ Sprectra convolution completed in 0.3207s


Protocol 1 completed in 3:24:59.701128
Active: 45 | Deactivated: 23

======================================================================
                            END PROTOCOL 1                            
======================================================================


======================================================================
                           PROTOCOL 2 - SP                            
======================================================================
Level: wB97X-D4rev/def2-QZVPP
Active conformers: 45
======================================================================
001. โ†’ CONF 001 | Protocol 2
	โœ“ E = -685.31971450 Eh | Time: 110.9s
002. โ†’ CONF 003 | Protocol 2
	โœ“ E = -685.31799534 Eh | Time: 120.5s
[...]

Total elapsed time for protocol 2: 1:25:40.446387

**********************************************************************
                        Summary Before Pruning                        
**********************************************************************

Conformers    E [Eh]             G-E [Eh]             G [Eh]              B [cm-1]    โˆ†G [kcal/mol]    Pop [%]    Elap. time [sec]    # Cluster
------------  -----------------  -------------------  ------------------  ----------  ---------------  ---------  ------------------  -----------
1             -685.319714502616  0.11078618968633691  -685.2089283129296  0.05793     nan              nan        110.92
3             -685.317995339016  0.10999790498283346  -685.2079974340331  0.05984     nan              nan        120.50
4             -685.315682557446  0.11032647075694513  -685.2053560866891  0.05627     nan              nan        111.97
[...]


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
       Pruning sequence       
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Filtering conformers above 6 kcal/mol energy window
โœ“ No conformers above threshold

No conformers deactivated by similarity check

โœ“ Pruning completed in 0.0067s
Initial: 45 โ†’ Final: 45 (100.0% retained)
Deactivated: 0


**********************************************************************
                        Summary After Pruning                         
**********************************************************************

Conformers    E [Eh]             G-E [Eh]             G [Eh]              B [cm-1]    โˆ†G [kcal/mol]    Pop [%]    Elap. time [sec]    # Cluster
------------  -----------------  -------------------  ------------------  ----------  ---------------  ---------  ------------------  -----------
9             -685.32009796484   0.11044178749125422  -685.2096561773487  0.06062     0.00             16.69      109.23
36            -685.319769173968  0.11034965162559729  -685.2094195223424  0.06045     0.15             12.99      108.83
1             -685.319714502616  0.11078618968633691  -685.2089283129296  0.05793     0.46             7.72       110.92
21            -685.318903971546  0.11001511261474661  -685.2088888589312  0.06079     0.48             7.40       118.59
43            -685.318371479997  0.10996062930803419  -685.208410850689   0.05831     0.78             4.46       111.15
8             -685.318510425123  0.11014181074847329  -685.2083686143745  0.05792     0.81             4.27       109.10
35            -685.318552897217  0.11042692433477441  -685.2081259728823  0.05780     0.96             3.30       110.28
3             -685.317995339016  0.10999790498283346  -685.2079974340331  0.05984     1.04             2.88       120.50
24            -685.318332712426  0.11046737228207348  -685.2078653401439  0.05812     1.12             2.50       106.48
49            -685.317398554083  0.10956784819984478  -685.2078307058831  0.05524     1.15             2.41       120.04
58            -685.317890824932  0.11011943635026     -685.2077713885817  0.05822     1.18             2.27       108.50
29            -685.318567404048  0.11084570170544339  -685.2077217023426  0.05746     1.21             2.15       121.59
80            -685.317960198245  0.1102592158420066   -685.207700982403   0.05806     1.23             2.10       120.31
10            -685.317519593746  0.10985834318285015  -685.2076612505632  0.05946     1.25             2.02       106.78
19            -685.318930543435  0.1113123708872763   -685.2076181725478  0.05786     1.28             1.93       108.20
30            -685.317076862248  0.10947887334168627  -685.2075979889063  0.05642     1.29             1.89       111.84
52            -685.318225593783  0.11062874927210942  -685.2075968445109  0.05765     1.29             1.88       113.13
23            -685.317620924531  0.11007877128463406  -685.2075421532463  0.05647     1.33             1.78       111.07
7             -685.317321928401  0.10980090270390974  -685.2075210256971  0.05519     1.34             1.74       121.66
13            -685.319519466402  0.11205136912985836  -685.2074680972721  0.05724     1.37             1.64       123.43
11            -685.319092822957  0.11168892862485791  -685.2074038943322  0.05911     1.41             1.53       113.75
22            -685.319225828119  0.11204399366101825  -685.207181834458   0.05704     1.55             1.21       121.37
47            -685.316838085898  0.10973288083448551  -685.2071052050635  0.05554     1.60             1.12       110.27
89            -685.31683994055   0.10977002015158632  -685.2070699203985  0.05580     1.62             1.08       121.62
5             -685.317118030773  0.11015522379409504  -685.2069628069789  0.05797     1.69             0.96       113.77
31            -685.318080206051  0.11112331937908948  -685.206956886672   0.05804     1.69             0.96       107.77
48            -685.317321241784  0.11053230150719173  -685.2067889402769  0.05965     1.80             0.80       118.38
20            -685.316779142598  0.11004969141072252  -685.2067294511872  0.05496     1.84             0.75       120.95
16            -685.318752120041  0.11202636599045945  -685.2067257540506  0.05896     1.84             0.75       110.68
15            -685.316934755705  0.11023221327775445  -685.2067025424273  0.05989     1.85             0.73       118.69
59            -685.316922428206  0.1102244057151438   -685.2066980224909  0.05976     1.86             0.73       121.78
42            -685.318682569395  0.11201324289334025  -685.2066693265017  0.05934     1.87             0.70       110.89
45            -685.317291132863  0.11063583374129848  -685.2066552991217  0.05639     1.88             0.69       112.28
55            -685.316737441477  0.1101439023674402   -685.2065935391096  0.05634     1.92             0.65       112.70
68            -685.316394322083  0.10991605524486658  -685.2064782668382  0.05509     1.99             0.58       122.24
6             -685.317487517239  0.11107219392408751  -685.2064153233149  0.05779     2.03             0.54       110.44
60            -685.318311427381  0.11200572215602733  -685.206305705225   0.05918     2.10             0.48       119.91
33            -685.316619617622  0.11035886185379695  -685.2062607557682  0.05943     2.13             0.46       118.90
39            -685.316987745366  0.11113315060663354  -685.2058545947593  0.05958     2.39             0.30       109.11
41            -685.317585530754  0.11173314480333829  -685.2058523859506  0.05768     2.39             0.30       112.64
4             -685.315682557446  0.11032647075694513  -685.2053560866891  0.05627     2.70             0.18       111.97
18            -685.316060278243  0.11071269882756951  -685.2053475794154  0.05566     2.70             0.17       108.55
112           -685.316318384567  0.11120082323486713  -685.2051175613321  0.05756     2.85             0.14       112.44
71            -685.315050364561  0.11008852790030232  -685.2049618366607  0.05462     2.95             0.12       110.37
51            -685.315491867108  0.11103599914235929  -685.2044558679656  0.05491     3.26             0.07       113.17

**************************************************
    Energetic Summary of the active conformers    
**************************************************

Conformer    โˆ†E [Eh]            Boltzamnn Pop. on โˆ†E    โˆ†(E+ZPVE) [Eh]      Boltzamnn Pop. on โˆ†(E+ZPVE)    โˆ†H [Eh]             Boltzamnn Pop. on โˆ†H    โˆ†G [Eh]             Boltzamnn Pop. on โˆ†G
-----------  -----------------  ----------------------  ------------------  -----------------------------  ------------------  ----------------------  ------------------  ----------------------
Conf 9       -685.32009796484   13.91                   -685.1717186063796  14.72                          -685.1594311169009  13.79                   -685.2096561773487  16.69
Conf 36      -685.319769173968  9.81                    -685.1714804865791  11.43                          -685.1591449748898  10.18                   -685.2094195223424  12.99
Conf 1       -685.319714502616  9.26                    -685.1712026231447  8.52                           -685.1590420098415  9.13                    -685.2089283129296  7.72
Conf 21      -685.318903971546  3.92                    -685.1708371549022  5.78                           -685.1583406187026  4.34                    -685.2088888589312  7.40
Conf 43      -685.318371479997  2.23                    -685.1703984782962  3.63                           -685.1579398337083  2.84                    -685.208410850689   4.46
Conf 8       -685.318510425123  2.59                    -685.1702495085975  3.10                           -685.157853843592   2.59                    -685.2083686143745  4.27
Conf 35      -685.318552897217  2.70                    -685.1702012640567  2.95                           -685.1579162616624  2.77                    -685.2081259728823  3.30
Conf 3       -685.317995339016  1.50                    -685.1699105020663  2.17                           -685.1575373481936  1.85                    -685.2079974340331  2.88
Conf 24      -685.318332712426  2.14                    -685.1699474762931  2.25                           -685.1576354486638  2.06                    -685.2078653401439  2.50
Conf 49      -685.317398554083  0.80                    -685.1695858898196  1.54                           -685.1570502154259  1.11                    -685.2078307058831  2.41
Conf 58      -685.317890824932  1.34                    -685.1697818394055  1.89                           -685.1574304422202  1.66                    -685.2077713885817  2.27
Conf 29      -685.318567404048  2.75                    -685.1704413322643  3.80                           -685.1584119473814  4.68                    -685.2077217023426  2.15
Conf 80      -685.317960198245  1.44                    -685.1697142037175  1.76                           -685.1573945129263  1.59                    -685.207700982403   2.10
Conf 10      -685.317519593746  0.90                    -685.1695503282401  1.48                           -685.1570762952053  1.14                    -685.2076612505632  2.02
Conf 19      -685.318930543435  4.04                    -685.1700359318025  2.47                           -685.1578692550653  2.63                    -685.2076181725478  1.93
Conf 30      -685.317076862248  0.57                    -685.1694392751667  1.31                           -685.1569876940923  1.04                    -685.2075979889063  1.89
Conf 52      -685.318225593783  1.91                    -685.1696279413006  1.61                           -685.1574301399087  1.65                    -685.2075968445109  1.88
Conf 23      -685.317620924531  1.01                    -685.1696454168457  1.64                           -685.1573331577849  1.49                    -685.2075421532463  1.78
Conf 7       -685.317321928401  0.73                    -685.1693612288053  1.21                           -685.156973041214   1.02                    -685.2075210256971  1.74
Conf 13      -685.319519466402  7.53                    -685.170599977179   4.50                           -685.1587191323391  6.48                    -685.2074680972721  1.64
Conf 11      -685.319092822957  4.79                    -685.1703414625049  3.42                           -685.1583630526692  4.45                    -685.2074038943322  1.53
Conf 22      -685.319225828119  5.52                    -685.1702743078591  3.19                           -685.158437664625   4.81                    -685.207181834458   1.21
Conf 47      -685.316838085898  0.44                    -685.1690014325295  0.83                           -685.1564965936312  0.62                    -685.2071052050635  1.12
Conf 89      -685.31683994055   0.44                    -685.1687651275379  0.64                           -685.1563950845541  0.55                    -685.2070699203985  1.08
Conf 5       -685.317118030773  0.59                    -685.1690804885833  0.90                           -685.1565646309797  0.66                    -685.2069628069789  0.96
Conf 31      -685.318080206051  1.64                    -685.1693155638816  1.15                           -685.1571035729041  1.17                    -685.206956886672   0.96
Conf 48      -685.317321241784  0.73                    -685.1689912739978  0.82                           -685.1567383599688  0.79                    -685.2067889402769  0.80
Conf 20      -685.316779142598  0.41                    -685.1685720591735  0.52                           -685.1562020458093  0.45                    -685.2067294511872  0.75
Conf 16      -685.318752120041  3.34                    -685.1697678852942  1.86                           -685.1578634114076  2.62                    -685.2067257540506  0.75
Conf 15      -685.316934755705  0.49                    -685.1688140831785  0.68                           -685.1564311559802  0.57                    -685.2067025424273  0.73
Conf 59      -685.316922428206  0.48                    -685.1688078383871  0.67                           -685.1564185812559  0.57                    -685.2066980224909  0.73
Conf 42      -685.318682569395  3.10                    -685.1697174256929  1.77                           -685.1578089125907  2.47                    -685.2066693265017  0.70
Conf 45      -685.317291132863  0.71                    -685.1689587957824  0.79                           -685.1567711428752  0.82                    -685.2066552991217  0.69
Conf 55      -685.316737441477  0.40                    -685.1686838382055  0.59                           -685.1563594858502  0.53                    -685.2065935391096  0.65
Conf 68      -685.316394322083  0.27                    -685.1683388962774  0.41                           -685.1559375957805  0.34                    -685.2064782668382  0.58
Conf 6       -685.317487517239  0.87                    -685.1688186492366  0.68                           -685.1565522655125  0.65                    -685.2064153233149  0.54
Conf 60      -685.318311427381  2.09                    -685.1693513184292  1.20                           -685.1574393074093  1.67                    -685.206305705225   0.48
Conf 33      -685.316619617622  0.35                    -685.1683567190906  0.42                           -685.1560337379696  0.38                    -685.2062607557682  0.46
Conf 39      -685.316987745366  0.52                    -685.1681745326628  0.34                           -685.1561059462815  0.41                    -685.2058545947593  0.30
Conf 41      -685.317585530754  0.97                    -685.1686880030669  0.59                           -685.1565785931786  0.67                    -685.2058523859506  0.30
Conf 4       -685.315682557446  0.13                    -685.1674997820701  0.17                           -685.1551437824294  0.15                    -685.2053560866891  0.18
Conf 18      -685.316060278243  0.19                    -685.1677560765326  0.22                           -685.1555225448094  0.22                    -685.2053475794154  0.17
Conf 112     -685.316318384567  0.25                    -685.1675085435519  0.17                           -685.1554272777529  0.20                    -685.2051175613321  0.14
Conf 71      -685.315050364561  0.07                    -685.1670273798624  0.10                           -685.154649410797   0.09                    -685.2049618366607  0.12
Conf 51      -685.315491867108  0.11                    -685.1670425689015  0.10                           -685.1547492836457  0.10                    -685.2044558679656  0.07

**************************************************
            Ensemble Average Energies             
**************************************************

T [K]    E_av [Eh]           E+ZPVE_av [Eh]      H_av [Eh]           G_av [Eh]
-------  ------------------  ------------------  ------------------  ------------------
298.15   -685.3189396413827  -685.1704615681656  -685.1582465923802  -685.2083572750538

----------------------------------------
          Spectra convolution           
----------------------------------------
โŠ— No calculation of IR graphs. Skipping
โŠ— No calculation of VCD graphs. Skipping
โŠ— No calculation of UV graphs. Skipping
โŠ— No calculation of ECD graphs. Skipping

โœ“ Sprectra convolution completed in 0.0848s


Protocol 2 completed in 1:25:40.547515
Active: 45 | Deactivated: 0

======================================================================
                            END PROTOCOL 2                            
======================================================================


======================================================================
                        Calculation COMPLETED                         
======================================================================
Total elapsed time: 4:52:39.33862
Final conformers: 45
======================================================================

**********************************************************************
                 CALCULATIONS COMPLETED SUCCESSFULLY                  
**********************************************************************

The protocol effectively reduces the number of structures by identifying duplicates and high-energy conformers.

The table below summarizes the retention rates achieved using this protocol on various flexible molecules, demonstrating the efficiency of the pruning strategy (results from Table 1 of the manuscript).

Molecule

Starting Ensemble

Protocol 1
g-xTB

Protocol 2
r\(^2\)SCAN-3c

Protocol 3
ฯ‰B97X-D4rev/def2-QZVPP

Final Retention Rate

Diethyl Phthalate

156

151

35

35

22 %

Guaiol

118

109

34

34

29 %

Penicillin V

136

71

12

12

9 %

Permethrin

304

304

101

101

33 %

Rivaroxaban

110

110

30

30

27 %

Sitagliptin

428

343

142

142

33 %

Tamiflu

836

527

100

99

12 %

Ascorbic acid

134

68

45

45

33 %